Project Description

Author: Hasan et al.

Summary:

In addition to the well-known underlying pathophysiology of nonalcoholic fatty liver disease (NAFLD), dysbiosis of the gut microbiota is a relatively newer concept. A lack of evidence considering human subjects is evident in our country. Therefore, finding the pattern of gut microbiota in NAFLD patients is the objective of the study. This cross-sectional study will be conducted at the Department of Medicine and Department of Hepatology, Dhaka Medical College Hospital (DMCH), for a period of six months. Formal ethical permission will be sought from the proper authority. Biopsy-proven NAFLD cases will be included. The severity will be defined by the NAFLD activity score (NAS). To identify gut dysbiosis, a metagenomic approach will be followed. In this process, the microbial community will be profiled by denaturing gradient gel electrophoresis and examined by 454 pyrosequencing of the 16S ribosomal RNA V3 region from a stool sample. For the convenience of the study, a total of 30 cases will be included in this study. A written informed consent form will be ensured before participation as well as for the biopsy procedure. The ethical measures will be taken in accordance with the current Declaration of Helsinki. Data analysis will be performed following completion and verification of the data, and the statistical software SPSS will be used during data analysis. It is expected that the result will prove the change in the composition of the gut microbiota in NAFLD patients and establish the relation of gut dysbiosis with the severity of NAFLD. Moreover, it will help clinicians and policymakers establish guidelines to prevent the increasing number of NAFLD cases in our country.

Status: Ongoing

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